Expression data from HPV 11E6 and HPV18E6 infected cells

The aim of this study was to compare the roles of the E6 proteins from HPV18 and HPV11, a high-risk and low-risk type, and to evaluate the effect of these two types of E6 proteins on gene expression We used microarrays to detail the gene expression patterns during the early stages of cellular transformation HPV 11 and HPV 18 E6 genes were cloned into adenoviral vectors for transformation of HaCaT cell lines to induce intense, high-level expression of these genes. RNA isolated from these cells were used in DNA microarray analysis to study and compare the effects that the E6 gene from the low-risk and high-risk type HPVs have on gene expression patterns during the early stages of transformation.Once data had been collected from the microarray study, further analysis of important genes and pathways was done by qRT-PCR and western blotting. Signalling pathways involved in E6-mediated transformation were identified, and the effect of inhibiting these pathways was investigated.