Metadata record for the article: Signaling of MK2 Sustains Robust AP1 Activity for Triple Negative Breast Cancer Tumorigenesis through Direct Phosphorylation of JAB1

<b>Summary</b><br>

This
metadata record provides details of the data supporting the claims of the
related article: “Signaling of MK2 Sustains Robust AP1
Activity for Triple Negative Breast Cancer Tumorigenesis through Direct
Phosphorylation of JAB1”.

The
related study showed that p38MAPK signalling pathway regulation of activator
protein 1 (AP1) activity involves both MAPKAPK2 (MK2) and JAB1, a known JUN
binding protein.

Type of data:
signalling pathway activity

Subject of
data: antibodies; Eukaryotic cell lines (ATCC); <i>Mus musculus</i> (Foxn1nu, female, Catalog# 007850, The Jackson
laboratory)

<b>Data
access</b>

The
following files underlying the figures in the related manuscript are openly
available with this data record:

- Fig.1
data.xlsx (Fig.1a,1b of the related article)

- Fig.2
data.xlsx (Fig.2c,2d
and 2e)

- Fig.3
data.xlsx (Fig.3e and
3f)

- Fig.4
data.xlsx (Fig.4f,4g and 4f)

- Fig.5
data.xlsx (Fig.5b,5c and 5d)

- Fig.6
data.xlsx

- Fig.7
data.xlsx (Fig. 7e and 7f)

- Fig.8
data.xlsx (Fig.8a and 8c)

All other
data supporting the related study can be found in the supplementary information
file of the related article, and the corresponding author can make any materials
available upon request. Un-cropped gels and western blots for Fig. to Fig.5
were included in Supplementary Materials (Fig.S11).”

JAB1
expression in different breast cancer subtypes were downloaded from https://tcga.xenahubs.net/download/TCGA.BRCA.sampleMap/HiSeqV2.gz,
and https://tcga.xenahubs.net/download/TCGA.BRCA.sampleMap/BRCA_clinicalMatrix.gz.
For analysis of p38MAPK activity in breast cancer, Reverse Phase Protein Array
(RPPA) z score and corresponding clinical data from TCGA Breast Cancer Invasive
Carcinoma, PanCancer Atlas were first downloaded through cBioportal (https://www.cbioportal.org/).

<b>Corresponding author(s) for this study</b>

Shuang
Huang, Department of Anatomy and Cell Biology, University of Florida College of
Medicine, Gainesville, FL 32610. E-mail: [e-mail]<br>
<b></b>

<b>Study
approval </b>

University
of Florida Institutional Animal Care and Use Committee.