Transcriptional control of thermogenesis by early B cell proteins [RNA-seq]

Early B Cell Factor 2 (EBF2) is required for brown adipose tissue basal thermogenic gene expression; however, it was unknown whether chronic cold exposure normalized the expression of thermogenic genes in Ebf2 mutant mice. We examined the transcriptome of Ebf2 WT and mutant brown adipose tissue in mice housed at room temperature or one week of cold exposure. RNASeq was performed in control and Ebf2 mutant brown adipose tissue from adult mice acclimated to room temperature or 1 week of cold exposure.早期B细胞因子2（Early B Cell Factor 2，EBF2）是棕色脂肪组织基础产热基因表达所必需的；然而，慢性冷暴露是否可使Ebf2突变小鼠的产热基因表达恢复至正常水平，此前尚不明确。我们对饲养于室温环境或经为期1周冷暴露处理的小鼠的Ebf2野生型（Wild Type，WT）与突变型棕色脂肪组织的转录组进行了检测。本研究对适应室温环境或经为期1周冷暴露处理的成年小鼠的对照组及Ebf2突变型棕色脂肪组织开展了RNA测序（RNASeq）实验。