1The wild-type hTTP protein was purified from transfected human cells after in vivo radiolabeling with [32P]-orthophosphate. The protein was purified and digested by trypsin to completion. The phospho

Figshare2015-12-02 更新20

Proteomics-Based Tools for Evaluation of Cell-Free Protein Synthesis

无细胞蛋白质合成

质谱蛋白质组学

Cell-free protein synthesis (CFPS) has the potential to produce enzymes, therapeutic agents, and other proteins, while circumventing difficulties associated with in vivo heterologous expression. Howev

Figshare2017-10-17 更新00

A comparative study of synthetic winged peptides for absolute protein quantification

蛋白质定量

质谱蛋白质组学

A proper internal standard choice is critical for accurate, precise, and reproducible mass spectrometry-based proteomics assays. Synthetic isotopically labeled (SIL) proteins are currently considered

NIAID Data Ecosystem40

Multi-Reference Spectral Library Yields Almost Complete Coverage of Heterogeneous LC-MS/MS Data Sets

质谱蛋白质组学

光谱库优化

Spectral libraries play a central role in the analysis of data-independent-acquisition (DIA) proteomics experiments. A main assumption in current spectral library tools is that a single characteristic

NIAID Data Ecosystem10