Crystal structure of the C. elegans POFUT2 (CePoFUT2) triple mutant (R298K-R299K-A418C) in complex with the Rattus norvegicus TSR4 single mutant (E10C) from F-spondin

Crystal structure of the C. elegans POFUT2 (CePoFUT2) triple mutant (R298K-R299K-A418C) in complex with the Rattus norvegicus TSR4 single mutant (E10C) from F-spondin Descriptor: 1,2-ETHANEDIOL, 2-acetamido-2-deoxy-beta-D-glucopyranose, GDP-fucose protein O-fucosyltransferase 2,Spondin-1, ... Authors: Hurtado-Guerrero, R, Merino, P. Deposit date: 2022-09-01 Release date: 2022-10-26 Last modified: 2024-10-09 Method: X-RAY DIFFRACTION (2.13 Å) Cite: The Essential Role of Water Molecules in the Reaction Mechanism of Protein O-Fucosyltransferase 2. Angew.Chem.Int.Ed.Engl., 61, 2022

秀丽隐杆线虫（C. elegans）POFUT2（CePoFUT2）三重突变体（R298K-R299K-A418C）与源自F-spondin的褐家鼠（Rattus norvegicus）TSR4单突变体（E10C）形成复合物的晶体结构。描述项：1,2-乙二醇、2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖、GDP-岩藻糖蛋白O-岩藻糖基转移酶2（GDP-fucose protein O-fucosyltransferase 2）、脊髓素-1（Spondin-1）…… 作者：Hurtado-Guerrero, R、Merino, P。沉积日期：2022-09-01；发布日期：2022-10-26；最后修改日期：2024-10-09。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率2.13埃（Å）。引用文献：《水分子在蛋白质O-岩藻糖基转移酶2反应机制中的核心作用》，《Angew.Chem.Int.Ed.Engl.》，61卷，2022年。