Mycorrhizal types of Chilean flora: unpublished data and grey literature

Contact: Dr. César Marín ([e-mail])We present the mycorrhizal type (arbuscular mycorrhizal, ectomycorrhizal, ericoid mycorrhizal, orchid mycorrhizal, non mycorrhizal) of 254 plant species found in Chile (with a total of 299 occurrences), belonging to 89 botanical families. Each occurrence contains information on: plant habit (tree, shrub, perennial herb, etc.), origin (native or introduced), site name, Chilean administrative region, collection date, coordinates, and mycorrhizal type. The mycorrhizal type data comes from three sources (the last two constitute 'grey literature'):1. Stained roots located at Soil Ecology Laboratory, Universidad Santo Tomás, Máfil, Chile; this data has not been published elsewhere. 2. Bachelor thesis: Flores Castro, R.A. 1995. “ESTUDIOS BASICOS DE LA RIZOSFERA PARA LA RESTAURACION DE LOS BOSQUES DE RUIL (Nothofagus alessandrii Esp.)”. Forestry Engineering, Austral University of Chile, Valdivia, Chile. Supervisor: Dr. Roberto Godoy B. 3. Conference proceedings: Godoy, R., Vogel, A., Carrillo, R., Hildebrand-Vogel, R. 1994. Zur Bedeutung der Mykorrhiza im Fitzroya cupressoides-Wald Südchiles. Verhandlungen der Gesellschaft für Ökologie 23: 135-141. Methods.For recording the flora and preparing herbarium specimens: Only flowering specimens were collected to obtain suitable material for herbarium deposits and formal taxonomic identification. Preliminary species identification was performed using relevant taxonomic literature. For root collection, species were identified in the field through in situ recognition. The updated flora nomenclature is based on: Rodriguez, R., Marticorena, C., Alarcón, D., Baeza, C., Cavieres, L., Finot, V. L., ... & Marticorena, A. (2018). Catálogo de las plantas vasculares de Chile. Gayana Botánica, 75(1), 1-430. Mycorrhizal type: Root samples from five plants of each vascular species were collected and stored in labeled nylon bags. These were then transported to the laboratory and, using clean forceps, placed in airtight plastic jars for fixation in 50% alcohol. The fixed and stained roots were processed and subsequently examined under a stereomicroscope and an optical microscope to visualize the different types of mycorrhizae. Acknowledgements.The National Forest Corporation of Chile (CONAF) for support across different National Parks, the Center for Research in the Dynamics of High-Latitude Marine Ecosystems (Centro IDEAL) (FONDAP No. 15150003) of Austral University of Chile, for laboratory facilities in Punta Arenas. We also thank the ANID-Chile' Fondecyt Regular Projects No. 1190642 (R.G.) and No. 1240186 (C.M.), and the VIDCA-UACh Project No. 43353934. (PI: Dr. Pablo Corti) for financial support. Many thanks to Benjamín Cáceres (Natural History Museum of Río Seco, Punta Arenas), Patricio Ojeda-González (RIP), Nicolás Pacheco, and Erwin Guzmán Riffo for fieldwork and laboratory support.