Gene expression signatures before or after transient middle cerebral artery oclussion (tMCAO) in ZEB1 transgenic and wild type mice. Gene expression signatures before or after transient middle cerebral artery oclussion (tMCAO) in ZEB1 transgenic and wild type mice

To further development of the role of ZEB1 in microglia, we have employed whole mRNA microarray expression profiling as a discovery platform to identify genes. Mouse microglia from wild type(wt), ZEB1-overexpression(ZEB1-tg) and ZEB1 knock down(ZEB1-kd) mice were isolated before and after tMCAO. The result showed after tMCAO, the wild type, ZEB1-overexpression and ZEB1 knock down microglia displayed a different gene profile, this suggested microglia ZEB1 might play an important role after ischemic stroke. Overall design: We first assessed the gene expressions in wild type, ZEB1-overexpression and ZEB1 knock down microglia before tMCAO. Then we measured the gene expressions in wild type, ZEB1-overexpression and ZEB1 knock down microglia after tMCAO.