Primers and PCR conditions used for the detection of target genes in laying hens digesta, blood, tissues, and eggs samples.

1PCR conditions for ivr F & R included 1 cycle at 95°C for 5 min, 40 cycles of 94°C for 30 s, down to 61.5°C for 45 s and back up to 72°C for 45 s and 1 cycle of 72°C for 10 min; phyA2-F & R; 1 cycle at 94°C for 5 min, 40 cycles of 94°C for 30 s, down to 61.5°C for 45 s and back up to 72°C for 45 s and 1 cycle of 72°C for 10 min; ov-F & R; 1 cycle at 94°C for 3 min, 35 cycles of 94°C for 30 s, down to 55°C for 30 s and back up to 72°C for 45 s and 1 cycle of 72°C for 10 min.