Doxorubicin Changes the Spatial Organization of the Genome around Active Promoters. Doxorubicin Changes the Spatial Organization of the Genome around Active Promoters

To investigate the function of the TOP2 inhibitors treatment in binding of chromatin architecture factors, we performed ChIP-seq. We used chromatin from RPE1 cells treated with four different conditions: control (DMSO-treatment), doxorubicin treatment (200 nM and 340 nM) and ICRF193 treatment (5uM). Overall design: Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for CTCF and RAD21.