Expression data form PI3K Knockout colon cancer cell lines (HCT116 and DLD1)

Whole transciptome analysis of colon cancer mutated cell lines(HCT116 and DLD1) under serum starvation conditions (19hrs-0.5%FBS) We used microarrays to compare gene regulation of truncated cell lines, knocked-out of either the wild type or mutated allele of PI3K, for two independent colon cancer cell lines Vogelstein's lab generated two truncated cell lines, knocked-out of either the wild type or mutated allele of PI3K, for two independent colon cancer cell liness (HCT116 and DLD1). These 4 cell lines were selected for RNA extraction under serum starvation conditions and RNA was used for hybridization on Affymetrix microarrays. We sought to identify potent regulators of carcinogenesis and metastases under these conditions