Supplementary tables S2 - S4_ and figure S1 - S7
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Supplementary Table S2. Summary of the taxonomic and geographic sampling of fungal genera for this study.
Supplementary Table S3. Specificity indices for Trebouxia OTUs represented by ≥ 10 sequences per OTU, based on fungal genera included in this study.
Supplementary Table S4. Summary of OTUs inferred using the ABGD detection program (Puillandre et al. 2012) represented by at least ten sequences and/or including sequences generated from UTEX and SAG cultures (UTEX and SAG cultures are shown in bold). Cultures and/or provisional names from previous studies, geographic distributions, and associated mycobiont hosts are reported. Other lichen mycobiont genera associated with Trebouxia OTUs were inferred using a BLAST search against the GenBank Nucleotide sequence database (searched 15 Nov 2014), where sequences with ≥ 98% similarity to a representative sequence from each OTU were assumed to belong to the OTU. The majority of OTUs were largely consistent with 97.5% sequence similarity clusters, with the exception of OTU ‘I01’, which was split into ten OTUs at 97.5%. The 97.5% sequence similarity clusters for OTUs ‘I01’ are shown parenthetically for each mycobiont host.
Supplementary Figure S1. Relative proportion of Trebouxia OTUs associated with sampled fungal genera
Supplementary Figure S2. ITS gene tree for a subset of specimens in the T. arboricola/gigantea group inferred using the program RAxML.
Supplementary Figure S3. Mitochondrial COXII gene tree for a subset of specimens in the T. arboricola/gigantea group inferred using the program RAxML.
Supplementary Figure S4. Chloroplast rbcL gene tree for a subset of specimens in the T. arboricola/gigantea group inferred using the program RAxML.
Supplementary Figure S5. Chloroplast psbJ-L gene tree for a subset of specimens in the T. arboricola/gigantea group inferred using the program RAxML. Ambiguous regions of the alignment were removed using the program Gblocks.
Supplementary Figure S6. Chloroplast topology for a subset of specimens in the T. arboricola/gigantea group inferred from concatenated rbcL and psJ-L alignments using the program RAxML.
Supplementary Figure S7. A “total evidence” phylogeny for the T. arboricola/gigantea, inferred from concatenated nuclear ITS, mitochondrial COXII, and the chloroplast markers rbcL and psbJ-L using the program RAxML.
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2015-06-11



