Hans Ris (2011) CIL:10708, Notophthalmus viridescens, oocyte. CIL. Dataset

Meiotic newt oocyte nuclei were allowed to disperse in 30 mM KCl/NaCl, centrifuged through 2% formaldehyde onto an EM grid, stained with uranyl acetate, and critical point dried. Grids were examined with the Madison 1MeV TEM at 10 KX. This micrograph, showing transcription on looped lampbrush chromosomes was recorded at a tilt angle of 45 degrees. The image is grouped with one of the same sample area recorded at a 55 degree tilt. A stereo examination of the pair provides an oblique 3D view.

在30 mM氯化钾/氯化钠溶液中，减数分裂蝾螈卵母细胞核被允许扩散，经2%甲醛固定后离心至电子显微镜铜网上，用铀酸乙酸染色，并在临界点干燥。铜网在10 KX条件下，使用Madison 1MeV透射电子显微镜进行观察。此显微照片展示了在螺旋梳状染色体上的转录过程，拍摄时倾斜角度为45度。该图像与在同一样本区域以55度倾斜角度拍摄的图像组群，通过立体观察这对图像可获得斜向的三维视图。