Phage-functionalized magnetic separation for rapid and selective detection of Escherichia coli

This dataset contains the raw data files used to generate all figures in the associated publication. Figure 1 (20250201.xlsx): Platereader export — OD₆₀₀ measurements of E. coli infection kinetics over 700 min at six different MOIs (10.70, 1.070, 0.107, 0.010, 0.001, 0.0001), a negative control, and an LB-broth blank. Triplicates per condition. Figure 6 (CMD-1000-Number-cum-1/2/3.txt, CMD-Phagen1.45-1000-Number-cum-1/2/3.txt, T4-Phagen-1000-Number-cum-1/2/3.txt): DLS number-weighted cumulative size distributions (3 replicates each) for ION@CMD, ION@CMD@Phage (phage-to-particle ratio 1.45), and free T4 phage. Tab-separated: diameter [nm], intensity [a.u.]. Figure 7 (20251002_CMD_EDCNHS_1_Ph_w.csv, 20251002_CMD_EDCNHS_1_Ph_R_w.csv): FT-IR transmittance spectra of CMD+EDC/NHS incubated with (+Phage) and without (−Phage, reference) T4 phage, both washed. Semicolon-separated; wavenumber [cm⁻¹], transmittance [%]. Figure S6 (20251001_CMD_1_w.csv): FT-IR transmittance spectrum of washed CMD particles. Figure S7 ([e-mail]): DLS number-weighted size distribution of sonicated ION@CMD (single measurement). Tab-separated: diameter [nm], intensity [a.u.]. Figure S9 (20251001_CMD_1_w.csv, 20251001_CMD_1_EDCNHS_w.csv): FT-IR transmittance spectra comparing washed CMD and washed CMD+EDC/NHS. Figure S10 (CMD.csv, CMD_Ph_W2.csv, CMD_Phage_W2.csv): FT-IR transmittance spectra of CMD, CMD+Phage (no EDC/NHS), and CMD+EDC/NHS+Phage (all wash fraction 2).