Yields of intact single haploid nuclei with the protocol.

Yield data from our laboratory using the protocol since optimisation. The data is from fresh and frozen testes, fresh epididymides, and cover wild-type and hypospermatogenic (Fancm-deficient) mice. “gated haploid nuclei” represents the total number of haploid nuclei that were gated using the strategy shown in Fig 2A–2F and isolated at gate “R6” (Fig 2F). “haploid percent of population (events pre-gating)” represents the number of haploid nuclei were gated (R6, Fig 2F) for a given experiment divided by the total number of events detected pre-gating. i.e. (R6 / events pre-gating) X 100.
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