RNA from A673 cells with shRNA-mediated knockdown of GFP (4 libraries), EWS-FLI1 (4 libraries), or lnc277 (7 libraries) was isolated with TRIzol (Invitrogen). Each sample was DNase treated and furth
We report the application of single-molecule-based sequencing technology for high-throughput profiling of bone marrow Ewing patients Overall design: Study of transcriptomic heterogeneity of Ewing Sarc