DNA Methylation-Based High-Resolution Mapping of Long-Distance Chromosomal Interactions in Nucleosome-Depleted Regions (4C)

Here, we present Methyltransferase Targeting-based chromosome Architecture Capture (MTAC), a method that maps the contacts between a target site (viewpoint) and the rest of the genome with high resolution and sensitivity. By targeting M.CviPI DNA methyltransferase to the viewpoint and by detecting differentially methylated regions, MTAC detects hundreds of intra- and inter-chromosomal interactions in the budding yeast genome that cannot be captured by 4C, Hi-C, or Micro-C.本研究介绍了基于甲基转移酶靶向的染色体构象捕获（Methyltransferase Targeting-based chromosome Architecture Capture，缩写MTAC）技术，该方法可高分辨率、高灵敏度地绘制靶位点（viewpoint）与基因组其余区域之间的染色质相互作用图谱。通过将M.CviPI DNA甲基转移酶靶向至该靶位点，并检测差异甲基化区域，MTAC可在酿酒酵母基因组中检测到数百种染色体内及染色体间的相互作用，而此类相互作用无法通过4C、Hi-C或Micro-C技术捕获。