Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Set1 S228A S228E mutant Transcriptomes

Cells need to coordinate gene expression with their metabolic states to maintain cell homeostasis and growth. However, how cells transduce nutrient availability to appropriate gene expression response via histone modifications remains poorly understood. Here,we reported cla4 catalyzed Set1 phosphorylaton regulates cell cycle and histone gene transcription. Overall design: Total RNA was isolated from exponential growing yeast cells by standard phenol-chloroform extraction procedures and the quality of RNA was examined using Agilent Bioanalyzer according to the manufacturer's instructions. Library construction, sequencing and bioinformatics analysis were performed . There are three biological replicates for WT and Set1 S228A, Set1 S228E mutants. Differential expression levels of aligned sequences were calculated using significant thresholds set at fold change over 1.5 and adjusted P-value < 0.05