Transcriptional profiling of Candida albicans SC5314, hgc1? and cph1?/efg1? in yeast-and hyphae-inducing conditions at two time points.

Goal of this study was to determine metabolic adaptation processes in C. albicans associated to hyphal morphogenesis. Accessory to the metabolic profiling the corresponding transcriptome was investigated. To identify media-specific and general adaptation three different hyphae stimuli were used (M199 pH 7.4, Human serum and N-Aectylglucosamine) were used and compared again two respective yeast conditions (SD and M199 pH 4). Two filamentation-affected mutant strains were included - lacking either two central regulators of filamentation (cph1?/efg1?) or a downstream effector (hgc1?) - for the identification of specfic morphotype-dependent metabolic adaptation in repsonse to hyphae stimuli. Two different time points (90 min and 240 min after transition to test medium) were investigated to define the progression of the examined adaptation processes. Overall design: C. albicans cells were cultivated in SD medium at 37°C until early log-phase was reached and transferred to the indicated medium, promoting either yeast or hyphae growth and incubated at 37°C for the indicated time.