Richard Allen (University of Hawaii) (2011) CIL:13128, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

High resolution image of quick-freeze deep-etch of the P-face of the contractile vacuole. This face has many IMPs that are presumably transmembrane proteins many of which have been pulled free of the leaflet. There are also pits in this leaflet which represent the IMPs that remain with the opposite leaflet. Although we have found no mechano-sensitive or voltage-gated channels in the CVC (Sugino et al., J. Exp. Biol. 208:3957-3969, 2005) it is clear that proton pumps are present in the decorated tubules and that protons are exchanged for other ions such as calcium, potassium and sodium as these ions can accumulate in the CV against a concentration gradient from the cytosol. Some of the IMPs may function as ion exchange channels. What caused the large holes is not known. The texture of the luminal contents resembles the CV content and indicates the membrane is indeed that of the contractile vacuole. TEM taken on 5/27/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

本数据集呈现了收缩性空泡的P面高分辨率快速冷冻深蚀图像。该面富含可能为跨膜蛋白的许多整合膜蛋白（IMP），其中许多已被从叶状结构中拉出。此外，该叶状结构中还存在一些凹坑，这些凹坑代表了与对面叶状结构相连的IMP。尽管我们未在收缩性空泡膜（CVC）中发现机械敏感性或电压门控通道（Sugino等人，实验生物学杂志，第208卷，第3957-3969页，2005年），但显而易见，质子泵存在于装饰性管状结构中，且质子与其他离子如钙、钾和钠进行交换，因为这些离子能够逆浓度梯度在细胞质中积累于收缩性空泡内。部分IMP可能作为离子交换通道发挥作用。至于造成大孔的原因尚不清楚。管腔内物质的纹理与收缩性空泡内容相似，这表明该膜确实属于收缩性空泡。该透射电子显微镜（TEM）图像由R. Allen于1992年5月27日使用80kV的Zeiss 10A拍摄，负片放大率为31,500倍。原始胶片使用Epson Perfection V750 Pro进行扫描。该图像最适用于定量分析。更多相关信息可访问（http://www5.pbrc.hawaii.edu/allen/）。