KEAP1 mutation in lung adenocarcinoma promotes immune evasion and immunotherapy resistance [scRNA-seq]

Immune cells were isolated from the lungs of mice bearing KEAP1 mutant or wildtype tumors. We found that in KEAP1 mutant tumors there is a reduction in CD103 DCs and early activated T cells as well as an increase in exhausted CD8 T cells in comparison to WT controls. KEAP1 mutant (n=1) or wildtype (n=1) murine KP lung cancer cells were injected IV into C57BL/6 mice. Lungs were harvested and extravascular CD45 cells were sorted for scRNA-seq. Samples were stained with hashtag antibodies. Libraries were generated for gene expression, hashtags, and TCR sequencing and were then sequenced.