Quantitative Analysis of different NK cell types Transcriptomes. Quantitative Analysis of different NK cell types Transcriptomes

We compared transcriptome of different types of NK cells including obtained from peripheral Blood (PB-NK), derived from human ES cell, H9-NK, derived from human iPSC (iPSC-NK), derived from CD34+ cells from umbilical cord blood (UCB-NK), NKcell line (NK92) and 3 cancer cell lines (K562, Raji and Jurkat). When comparing the similarity of the NK cells populations by the number of differentially expressed genes to iPSC NK cells, there were the fewest differentially expressed genes in the comparison with the UCB NK cells, followed by the PB-NK cells, followed by the H9 embryonic stem cell derived-NK cells and lastly by the NK92 cell line. Overall design: mRNA profiles of 5 different NK cell type and 3 cancer cell lines were generated by deep sequencing, in dulpicate, using Illumina Hi-seq 2000.

搜集汇总

背景与挑战

背景概述

该数据集比较了五种不同来源的NK细胞（外周血、人胚胎干细胞、诱导多能干细胞、脐带血、NK92细胞系）和三种癌细胞系的转录组。通过差异表达基因分析，揭示了iPSC来源NK细胞与脐带血来源NK细胞在转录组上最为相似，其次为外周血NK细胞，而NK92细胞系差异最大。数据由Illumina Hi-seq 2000测序产生，共16个SRA实验。