Single-cell profiling reveals altered immune landscape and impaired NK cell function in gastric cancer liver metastasis

Gastric cancer (GC) is a substantial global health concern, and the development of liver metastasis (LM) in GC represents a critical stage linked to unfavorable patient prognoses. In this study, we employed single-cell RNA sequencing (scRNA-seq) to investigate the immune landscape of GC liver metastasis, revealing several immuno-suppressive components within the tumor immune microenvironment (TIM). We employed scRNA-seq to comprehensively analyze the immune landscape in gastric cancer liver metastasis. We collected tissues from three liver metastases (LM) of gastric cancer, along with matched primary GC samples. Additionally, we included three liver samples from healthy donors (HL) for comparison.