DUX4-r neomorphic activity depending on GTF2I in acute lymphoblastic leukemia [CUT&Tag]

Translocations producing rearranged versions of the transcription factor DUX4 (DUX4-r) are one of the most frequent causes of B-cell acute lymphoblastic leukemia (B-ALL). DUX4-r retains the DNA binding domain of wt DUX4, but is truncated on the C-terminal transcription activation domain. The precise mechanism through which DUX4-r causes leukemia is unknown and no targeted therapy is currently available. We found that the rearrangement leads to both a loss and a gain of function in DUX4-r. Loss of CBP/EP300 transcriptional co-activators interaction and inability to bind and activate repressed chromatin. Gain of interaction with the transcription factor GTF2I, which redirects DUX4-r toward leukemogenic targets. Importantly, this neomorphic activity exposes an Achilles' heel whereby DUX4-r positive leukemia cells are exquisitely sensitive to GTF2I targeting, which inhibits DUX4-r leukemogenic activity. Our work elucidates the molecular mechanism through which DUX4-r causes leukemia and suggest a possible therapeutic avenue tailored to this B-ALL subtype. Overall design: CUT&Tag of WT DUX4 and DUX4-r variants REH cells. CUT&Tag on H3K27Ac and CUTAC (chromatin accessibility profiling) in REH cells expressing WT DUX4 and DUX4-IGH CUT&Tag of WT DUX4 and DUX4-IGH in HEK cells transfected with EV or GTF2I. CUT&Tag of GTF2I in HEK cells, DUX4-IGH expressing and EV control expressing REH cells Please note that the processed data files generated from both rep1 and rep2 samples are linked to the corresponding *rep1 sample records.