Equal parental contribution to the transcriptome is not equal control of embryogenesis. Equal parental contribution to the transcriptome is not equal control of embryogenesis

The goal of present study is to investigate parental contributions to the transcriptome of apical and basal cell lineages in early embryogenesis. We found equivalent parental genome derived reads in the transcriptome of apical and basal cell lineages of early proembryos. Hundreds of parent-of-origin genes were identified in both apical and basal cell lineages according to our allele- specific transcriptome data. Overall design: To determine parental contributions to apical and basal cell lineages, the transcriptome of apical and basal cell lineages of early proembryos at 1-cell and 32-cell embryo stages derived from the crosses between the Col-0 and Ler were sequenced. To minimize the variance and avoid the influence of inherent allele-specific expression in the transcriptome, three independent biological replicates were performed for each hybrid cell type.