Tatyana M. Svitkina, Alexander B. Verkhovsky,, Gary G. Borisy (2011) CIL:23038, Rattus, fibroblast. CIL. Dataset

An REF-52 cytoskeleton with actin filaments decorated by myosin S1. An intermediate filament associates with an actin filament bundle via plectin sidearms (10-nm immunogold labeling). Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 10a from J Cell Biol. 1996 Nov;135(4):991-1007. Image corresponds to Fig 10a in J Cell Biol. 1996 Nov;135(4):991-1007.

本数据集展示了一幅REF-52细胞骨架，其肌动蛋白丝被肌球蛋白S1所修饰。中间丝通过plectin侧臂与肌动蛋白丝束相连接（10纳米免疫金标记）。细胞骨架的电子显微镜成像技术依照Svitkina等（1995年）所述方法进行。简言之，盖玻片上的细胞被如光镜检查那样裂解，经重组凝胶素N端域处理，以戊二醛、鞣酸和铀酸乙酯固定，临界点干燥，并镀铂碳。图像对应于《细胞生物学杂志》1996年11月第135卷第4期的图10a。图像对应于《细胞生物学杂志》1996年11月第135卷第4期的图10a。