Transcriptome sequencing analysis unravel the changes in osteoclast transcription after duloxetine hydrochloride intervention

Osteoclasts play an important role in the maintenance of bone homeostasis. Excessive formation and activation of osteoclasts can accelerate bone loss, thereby increasing the risk of osteoporosis and even fractures. In this study, duloxetine hydrochloride was found to inhibit osteoclast differentiation during in vitro culture.We extracted bone marrow cells from the femur and tibia of 6-8 week-old mice and induced them into osteoclasts in vitro with MCSF and RANKL. The experimental group was intervened with 5uM duloxetine hydrochloride. When osteoclast fusion reached maximum in the control group, cells were lysed with Trizol and stored at -80 degrees refrigerator. All experiments were performed three times independently. By transcriptome sequencing, we found that duloxetine hydrochloride could induce transcriptional changes during osteoclast differentiation. Through differential gene analysis, we found that the expression of osteoclast-related genes (Ctsk, Mmp9, Nfatc1, Dcstamp, Acp5, Atp6v0d2) was significantly down-regulated after duloxetine hydrochloride treatment.