Chemical capping improves template switching and enhances sequencing of small RNAs. Chemical capping improves template switching and enhances sequencing of small RNAs

We deployed CapTS-seq for sequencing synthetic miRNAs, human total brain RNA, and liver FFPE RNA, and demonstrated that chemical capping in conjunction with template switching consistently reduces sequencing bias and improves library quality in comparison with commercially available RNA-seq kits. Finally, we showed the simultaneous detection of miRNAs and mRNAs in FFPE derived samples, underscoring the potential of this workflow for dissecting regulatory networks between miRNAs and their target gene transcripts. Overall design: Examined small RNA and transcriptome content in synthetic miRNAs and total brain RNA and liver FFPE RNA using either CapTS-seq or commercially available SMARTer smRNA Seq Kit from Takara.