Mulberry, an edible plant, bark derived exosomes-like nanoparticles (MBELN) treatment of B6 mice for 7 days [Crypt]

MBELN isolated mulberry bark purified by sucrose gradient were orally gavaged to B6 mice for 7 days once in a day at aconcentration of 10 x 10E+09 particle/100 µl. After 7 days, colon specific crypt cells were isolated. RNA was isolated and sent to invitorgen for Affymetrix array. The purpose of the experiment was to see which set of genes go up or down after MBELN treatment and their link to gut homeostasis. We used affymetric array to see differential gene expression after the MBELN treatment in B6 mice colon specific crypt.