Single-cell RNA-Sequencing of pancreatic and PDAC stromal cells

Most cancers including pancreatic ductal adenocarcinoma (PDAC) are infiltrated by PNS neurons participating in their complex tumor microenvironment. However, their cell bodies and nuclei are located in the para- and pre-vertebral PNS ganglia located far from the tumor mass itself. Thus, molecular information on healthy organ- vs. cancer-infiltrating neurons is currently lacking in any sequencing dataset of healthy or tumor tissue. Here, we specifically isolate fibroblasts, immune, endothelial and PDAC cells and subject them to single-cell RNA-Sequencing using the 10x genomics platform.包括胰腺导管腺癌（pancreatic ductal adenocarcinoma, PDAC）在内的多数癌症，其肿瘤微环境中均有外周神经系统（Peripheral Nervous System, PNS）神经元浸润，并参与构成复杂的肿瘤微环境。然而，此类神经元的胞体与细胞核位于远离肿瘤本体的椎旁及椎前外周神经节中。因此，当前健康组织或肿瘤组织的各类测序数据集，均缺失健康器官来源与癌症浸润来源神经元的分子信息。本研究中，我们特异性分离成纤维细胞、免疫细胞、内皮细胞及胰腺导管腺癌细胞，并借助10x Genomics平台对其开展单细胞RNA测序。