Transcriptome analysis of cuprizone mouse model following AAV-mediated delivery of A Proliferation Inducing Ligand

A Proliferation Inducing Ligand (APRIL) is a member of the tumour necrosis factor (TNF) superfamily and has recently been shown to modulate pro-inflammatory astrocyte responses, as well as to ameliorate disease outcome in the experimental autoimmune encephalomyelitis mouse model for multiple sclerosis (MS). We demonstrated that adeno-associated virus (AAV)-mediated delivery of APRIL was able to rescue immune-stimulated murine iPSC-derived neurospheroids from detrimental cell death and neurodegenerative processes. In addition, T2 and diffusion weighted MRI with histological confirmation demonstrated that AAV-mediated secretion of APRIL by cortical neurons could reduce cuprizone-induced neuro-inflammation and/or demyelination in the splenium of the corpus callosum. To further document the beneficial effect of APRIL, a transcriptome-proteome integration study for the splenium was performed that revealed the activation of cellular pathways associated with alternative immune cell polarisation, cell survival and neuroregeneration. Here, the associated transcriptome data (FASTQ files and BAM files) are deposited.