False tracking rate of selected MCF-10A, MCF-7, and MDA-MB-231 cells.

aTotal tracking event is the sum of the all cell-tracking events over the total selected cells that underwent an entire cell cycle. bFalse association is mostly due to false detection of cell division, rapid change of cell locations among multiple interacting cells. cLost tracking is mainly due to low image contrast, abrupt motion due to rapid contraction of focal adhesion. Note that cells moving out the field-of-view were not counted as false tracking events.