Survey sequencing of bread wheat chromosome 5D

We generated shotgun sequences from each arm of flow-sorted Triticum aestivum chromosome 5D using 454 FLX Titanium technology, giving 1.34x and 1.61x coverage of the short (5DS) and long (5DL) arms of the chromosome respectively. By a combination of sequence similarity and assembly-based methods, ~74% of the sequence reads were classified as repetitive elements, 1493 (5DS) and 2829 (5DL) conserved genes were identified, and 6312 putative lineage-specific or genes or gene fragments were also detected.