PHLOWER - Single cell trajectory analysis using Decomposition of the Hodge Laplacian [multiome]

To dissect nephrogenic differentiation trajectories in kidney organoids using the multiome pipeline, organoids were harvested at different time points during culture. Overall design: The following differentiation stages were processed: day 7 (cells harvested from the 2D cell layer, primitive streak - intermediate mesoderm stage), organoids day 7+5~(day12), day 7+12~(day19) and day 7+18~(day25).