Sex differences in the developmental patterns of the cortical astroglial cell translatome

Employing translating ribosome affinity purification with RNA sequencing (TRAPseq) on cortical tissue to isolate astroglial-specific translatome from Aldh1l1-L10-GFP mice and examine sex (male + female) and age (postnatal days 1, 4, 7, 14, 35; adults (9 weeks)) dependent changes in the astroglial translatome. Cortical lysate (N=53 independent samples) from male and female Aldh1l1-GFP reporter mice (C57BL/6 background strain) at 6 different postnatal time points was used for translating ribosome affinity purification (TRAP) to extract actively transcribed RNA and then used with Illumina Hi-Seq RNA sequencing. Four of the samples (P7 Female 10, P7 Female 6, P7 Male 2, P7 Male 6) were sequenced twice.