Employed three different RNAi methodologies in wild type N2 strain of C. elegans for uncoordinated phenotype.
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Method I, RNAi clone of unc-73 co-incuabted with RNAi enhancers (eri-1 and lin-35); Method II, Pre-incubation with eri-1 RNAi clone followed by incubation with unc-73 RNAi clone, co-incubation with eri-1 and unc-73 RNAi clones and co-incubation with unc-73, eri-1 and lin-35 RNAi clones; Method III, Pre-incubation with unc-73 RNAi clone followed by incubation with eri-1, co-incubation with unc-73 and eri-1 RNAi clones and co-incubation with unc-73, eri-1 and lin-35 RNAi clones. unc, uncoordinated; WT, wild-type. +,weak phenotype; ++, medium phenotype; +++, strong phenotype.
创建时间：
2015-12-02
