AL589863.1 inhibits the progression of pancreatic cancer through regulating miR-671-5p/THBS1 axis

We obtained mRNA and lncRNA expression profiles from 178 PC tissues and 4 normal pancreatic tissues in the TCGA database. We further downloaded the high throughput database of 167 normal tissues of the pancreas in the GTEx database. Then, the TCGA and GTEx datasets were integrated for further analysis. Three microarray datasets, including mRNA (GSE15471 and GSE62165) and miRNA (GSE32678), were downloaded from the GEO database. GSE15471 contained 39 pairs of pancreatic ductal adenocarcinoma tumors (PDAC) and adjacent normal tissues. GSE62165 included 118 PDAC and 13 adjacent normal samples. GSE32678 covered 25 PC samples and 7 adjacent normal samples. 

The Limma package in R was carried out to identify DEGs, DEmiRNAs, and DElncRNAs. We identified the DEGs with threshold values of |log 2 FC|> 1.5 and adjusted. p-value< 0.05. DEmiRNAs and DElncRNAs were determined with |log 2 FC|> 1.5/2.0 and p-value< 0.05. Additionally, volcano plots were drawn to better visualize these DElncRNAs, DEmiRNAs, and DEGs using R software.