Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM
High-density oligo libraries and CRISPR-Cas9 technology have revolutionized large-scale genetic screens, primarily in cell culture. Here, we present novel platforms for multicellular systems that util
Background: LincRNAs play critical roles in eukaryotic cells, but systematic analyses of lincRNAs of an animal for phenotypes have been missing. We have generated CRISPR knockout strains for C. elegan
