A Transcriptome Fingerprinting Assay for Clinical Immune Monitoring. A Transcriptome Fingerprinting Assay for Clinical Immune Monitoring

Development of a cost-effective and practical targeted transcriptional fingerprinting assay (TFA) for monitoring a diverse repertoire of immune responses identified in the human blood transcriptome relying on a modular transcriptional repertoire built on the basis of variations in transcript abundance measured on a genome wide scale in the blood of nearly 1000 subjects across 16 immunologic conditions. Overall design: Total of 985 samples across 16 datasets. Each dataset contains it's own biological controls, there are no replicates. Samples are distributed as follows: TB (23 condition, 11 control, 34 total); Staph aureus (99 condition, 44 control, 143 total); Sepsis (35 condition, 12 control, 47 total); HIV (28 condition, 35 control, 63 total); Flu (25 condition, 14 control, 39 total); RSV (70 condition, 14 control, 84 total); B-cell deficiency (20 condition, 13 control, 33 total); Liver Transplant (94 condition, 30 control, 124 total); Pregnancy (25 condition, 20 control, 45 total); Melanoma Stage IV (22 condition, 5 control, 27 total); Kawasaki (21 condition, 23 control, 44 total); Juvenile Dermatomyositis (40 condition, 9 control, 49 total); COPD (19 condition, 24 control, 43 total); MS - untreated (34 condition, 22 control, 56 total); Pediatric SLE (55 condition, 14 control, 69 total); SoJIA (62 condition, 23 control, 85 total)