New point mutations in the sodium channel gene and deciphering pyrethroid resistance using RNA-seq in Triatoma dimidiata from Colombia

This study focuses on determining resistance to different pyrethroids in populations of Triatoma dimidiata of Boyacá, Colombia. To determine the possible causes of resistance, we sequenced RNA of three populations: laboratory, field, and under pressure of lambda-cyhalothrin. Transcriptomic profile analyses of T. dimidiata revealed differentially regulated genes in both the field population and in the laboratory population under pressure from lambda-cyhalothrin. Gene enrichment analysis showed positive regulation of transcripts related to detoxifying enzymes and mitochondrial proteins, suggesting a significant role in resistance. Overall design: To determine changes in the mRNA profiles of Triatoma dimidiata, we selected three individuals from the field population, three from the first-generation lambda-cyhalothrin pressure population, and three susceptible laboratory individuals.