RNA-seq analysis of differentially expressed genes between WT and shTRIM21 HCT116 cells.

To investigate the biological function TRIM21 in the colorectal cancer, we established HCT116 cell lines in which the TRIM21 gene has been knocked down by shRNA. We used a lentiviral shRNA technique to knockdown TRIM21 expression in HCT116 cells. A scrambled shRNA (shRNA/Control) was used as a control. We then performed RNA-Seq with Cloud-seq Biotech Inc. (Shanghai, China) to analyze gene expression changes in HCT116 cells. For each sample, three independent biological replicates were performed. Comparative gene expression profiling analysis of RNA-seq data for HCT116 cells and its KD derivatives (shTRIM21).