Notopterygium incisum Ting ex H. T. Chang Raw sequence reads

Study Objectives and RelevanceThis study employs integrated multi-omics approaches to decipher the molecular mechanisms underlying the development of silkworm-like rings (SLRs) - a specialized morphological trait in the rhizome of Notopterygium incisum, a critically endangered alpine medicinal plant. SLRs serve as a key quality marker for high-grade "Can-Qiang" medicinal material, yet their genetic regulation remains unexplored.We generated strand-specific RNA-seq data from underground organs across critical developmental stages:Temporal dynamics: Pre-SLR formation (T1A, July) vs. SLR formation peak (T2A, August)Spatial specificity: SLR-bearing rhizomes (T2RH) vs. roots (T2RO)(Total samples: n=24, with 6 biological replicates per group; there are also three biological replicates of T3A.)The transcriptomic data reveal:- Environmental triggers of SLR morphogenesis through phosphatidylinositol signaling and NAC/ERF-mediated transcriptional networks- Carbon redirection from primary metabolism to phenylpropanoid biosynthesis, driving secondary xylem proliferation and cork layer formation- Organ-functional specialization: Rhizome-specific activation of starch metabolism for energy storage vs. root-enriched defense compound synthesisScientific Value:- First comprehensive transcriptome resource for N. incisum, addressing IUCN Red List concerns (Endangered species)- Identifies candidate genes (e.g., CHIT, PIP14, UXS4) for breeding SLR-enhanced varieties- Provides mechanistic insights into alpine plant adaptation through "structural-metabolic coevolution"Data Applications:- Reference for medicinal plant organ development (SRX accession: [pending])- Cross-species comparison with tuber/root crops (e.g., Ipomoea batatas, Solanum tuberosum)- Foundation for metabolic engineering of pharmaceutically active coumarins