Development of gene expression signatures for high glucose-induced renal tubular fibrosis. Development of gene expression signatures for high glucose-induced renal tubular fibrosis

To further explore differentially expressed genes in high glucose-induced renal tubular fibrosis cells, we used whole transcriptome microarray expression profiling as a discovery platform to identify genes with a potential role in the high glucose-induced renal fibrosis process. Low-glucose stimulation (5.5 mM glucose) and high-glucose stimulation (25 mM glucose) of human renal tubular HK2 cells identified a consensus profile of 644 highly expressed genes to distinguish high-glucose fibrotic renal tubules from control samples. Overall design: Gene expression was measured in fibrotic kidney tubules after exposure to 25 mM glucose for 72 hours. Three independent experiments were performed, each using a different batch.