Figure S2 - Incomplete Recovery of Pneumococcal CD4 T Cell Immunity after Initiation of Antiretroviral Therapy in HIV-Infected Malawian Adults

Functionality of CD4+ T cells after ART. Patients were analyses prior to initiation of ART and followed-up at 3, 6 and 12 mths ART for different combinations of IFN-γ, TNF-α and IL-2 using flow cytometry and SPICE software (version 4). Charts show the frequency of CD4+ T cells producing one, two or three cytokines specific for (A) wild-type Streptococcus pneumoniae strain concentrated culture supernatant (CCS) (D39WT CCS) (B) an isogenic pneumolysin (ply)-deficient mutant (D39Ply- CCS) (C) Influenza antigens (D) M. tuberculosis PPD. (TIF)