Effects of 4-1BB ligation in spleen cells of NP- and P-mice stimulated with Ag85B protein on expression of activation markers on CD4+ T cells.

Unfractionated spleen cells of P- or NP-mice were cultured with Ag85B protein (5 µg/ml) in the presence of IgG2a control Ab or agonist anti-4-1BB mAb (5 µg/ml). After 6 days of culture cells were labeled with the indicated mAbs. Cell surface molecule expression was analyzed by flow cytometry as described in Materials and Methods. The results are presented as mean ± SEM of the percentage of CD4+ T cells staining for the indicated markers. Pooled data of 3 independent experiments are shown.#, § statistical significance (p