Precision Chemoradiotherapy via EGFR Targeting with Radiotherapy-Activated Drug Conjugates

The strategy of concurrent chemoradiotherapy (CCRT) has
been developed,
aiming to leverage the benefits of chemotherapy and radiotherapy while
mitigating their respective limitations. In this study, we innovatively
employed an azobenzene structure as a pivotal group responsive to
X-ray irradiation, designing radiotherapy-triggered azobenzene linkers,
augmented with an antibody as a targeting moiety, to formulate radiotherapy-triggered
antibody-drug conjugates (RT-ADCs). These conjugates can precisely
target those tumor cells with high expression of the epidermal growth
factor receptor (EGFR), thereby accumulating at the tumor site. Upon
exposure to X-ray irradiation, the azobenzene linkers undergo cleavage,
resulting in the localized release of cytotoxic agents at the tumor
site, effectively eliminating tumor cells. In vivo assays demonstrate
that the tumors in the treatment group of mice nearly vanished, with
tumor growth inhibition (TGI) exceeding 90%, and median survival time
(MST) significantly extended. We are confident that RT-ADC holds substantial
clinical application potential and can profoundly influence the field
of deep-seated tumor therapy.