fowl adenovirus 4 (FAdV-4) transcriptome by RNA sequencing

To analyze FAdV-4 transcriptome, RNA was extracted from FAdV4-CX19A-infected chicken LMH cells at 8, 12 and 18 hours post infection (hpi). After poly(A) mRNA enrichment, samples were subjected into an RNA-seq pipeline for non-stranded 150 bp paired-end sequencing. HISAT2 was used to map RNA-seq reads to the virus genomes. To analyze the fowl adenovirus 4 (FAdV-4) transcriptome by deep sequencing, RNA samples were extracted from chicken LMH cells at 12, 18 or 26 h post FAdV-4 infection, and subjected to Illumina strand-specific RNA-seq or nanopore full-length PCR-cDNA sequencing. After removing the reads of host cells, the data of FAdV-4 nanopore full-length cDNAs (transcripts) were corrected with reads from the Illumina RNA-seq and mapped to the viral genome.