Horizontal Gene Transfer of SGI-4 or a plasmid containing oriTSGI-4 to Salmonella enterica serovar Typhimurium

Conjugation efficiency of Salmonella Genomic Island 4 (SGI-4) from donor Salmonella enterica serovar I 4,[5],12:i:- or a plasmid containing oriTSGI-4 to recipient strain Salmonella enterica serovar Typhimurium. The oriTSGI-4 nucleotide sequences are located in the region that spans nucleotides 4704600-4706000 of the Salmonella enterica serovar I 4,[5],12:i:- strain USDA15WA-1 genome (CP040686). Conjugation efficiency was calculated by dividing the colony forming units (CFU)/mL of transconjugants by the CFU/mL of donors. A transconjugant is a bacterial recipient that received DNA from the donor strain due to horizontal gene transfer.

研究内容为从供体沙门氏菌血清型I 4,[5],12:i:-或携带oriTSGI-4的质粒中提取的沙门氏菌基因组岛4（SGI-4）的转化效率，转化受体为沙门氏菌血清型Typhimurium。oriTSGI-4的核苷酸序列位于沙门氏菌血清型I 4,[5],12:i:-菌株USDA15WA-1基因组（CP040686）中跨越4704600至4706000碱基的区域。转化效率通过将转导子（由供体菌株因水平基因转移接收DNA的细菌受体）的菌落形成单位（CFU）/毫升与供体菌株的CFU/毫升之比进行计算得出。转导子是指通过水平基因转移接收供体菌株DNA的细菌受体。