Single Cell RNA-Seq data for Cancer Stem Like Reversal Project

Primary breast cancer samples were obtained from malignant pleural effusions or ascites after completely red blood cells depletion. For single cell RNA sequencing, frozen vials were thawed and viable breast cancer cells were obtained after depletion of dead cells and CD45+ cells. The purifed patient cells were cultured in Renaissance medium (3D) and treated with chemotherapy (doxorubicin 0.1 µM, carboplatin 0.1mM, paclitaxel 1µM) for 72 hours, and then replaced with Renaissance medium containing DMSO/belinostat (1µM) for 72 hours incubation. The cells were collected, purified with Dead Cell Removal Kit, and subjected to ICELL8® Single-Cell System. Chemotherapy and belinostat strongly reprogrammed transcriptome of patient cells for cancer stem-like promotion and reversal. In total, two patients samples (#5 and #6) with three chemothrapies plus belinostat/DMSO treatment sequentioally were analyzed. *** Raw data not provided due to patient privacy concerns. ***