Impact of gas plasma-induced chronic oxidative stress on head and neck cancer. Impact of gas plasma-induced chronic oxidative stress on head and neck cancer

We have employed whole genome microarray expression profiling to identify genes that are differentially expressed after a single gas plasma exposure in two different head and neck cancer cell lines. Additionally, we aimed to identify genes correlating with cellular adaptation upon repetitive oxidative stress wherefore two different head and neck cancer celllines were exposed to gas plasma weekly in eight treatment cycles. Overall design: Plasma induced gene expression in human head and neck cancer cells was measured 7 days following a single gas plasma exposure or 7 days following the eighth gas plasma exposure. Due to the high complexity and elaborateness of this study only one independet experiment was performed, but 48 technical replicates were pooled for sample collection.