O-antigen Deficiency Sparks Metabolic Shifts That Blunt ROS-Mediated Killing by Meropenem

This dataset contains fluorescence microscopy images acquired using ROS probe and the corresponding analysis scripts. The data capture intracellular ROS levels in wild-type (WT) and ΔwbbM strains under untreated and meropenem-treated conditions. Image-based and single-cell analyses show that WT cells exhibit higher ROS levels, while ΔwbbM mutants display reduced fluorescence, particularly after antibiotic exposure. These results indicate that O-antigen deficiency is associated with reduced ROS accumulation and carbapenem resistance.